Journal: Physiological Genomics

Article Title: Microarray analysis of aging-associated immune system alterations in the rostral ventrolateral medulla of F344 rats

doi: 10.1152/physiolgenomics.00131.2016

Figure Lengend Snippet: List of immune system genes used for quantitative PCR validation of microarray analysis and their TaqMan assay IDs

Article Snippet: Actb, Hprt1, Ldha , and Rplp1 genes were used as endogenous controls and the geometric average of their Ct values was used to calculate each gene’s ΔCt value ( 1 ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Functional Category Gene Symbol TaqMan Assay ID Complement system C1qa Rn01519903_m1 C1qc(C1qg) Rn01516757_m1 Cd93(C1qr1) Rn00584525_g1 C3 Rn00584525_g1 C4a Rn00566466_m1 Cfd Rn00709527_m1 Cfh Rn01535436_g1 Vwf Rn00590326_m1 Klkb1 Rn01488161_m1 Microglial cells Cd14 Rn00572656_g1 Cd68 Rn01495634_g1 Tlr2 Rn02133647_s1 Cx3cr1 Rn02134446_s1 Trem2 Rn01512170_m1 Fcrl2 Rn01455191_m1 B2m Rn00560865_m1 Endogenous controls Actb Rn00667869_m1 Hprt1 Rn01527840_m1 Ldha Rn00820751_g1 Rplp1 Rn03467157_gH Open in a separate window List of immune system genes used for quantitative PCR validation of microarray analysis and their TaqMan assay IDs Microarray Data Analysis Hybridization signal intensities from microarrays were extracted with Agilent Feature Extraction Software, version 9.5.1.1. (Agilent Technologies) (Gene Expression Omnibus accession number {"type":"entrez-geo","attrs":{"text":"GSE90956","term_id":"90956"}} GSE90956 ).

Techniques: Real-time Polymerase Chain Reaction, Biomarker Discovery, Microarray, TaqMan Assay

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Fabrication of Functional Cell Sheets with Human Thyrocytes from Non-Tumorous Thyroid Tissue

doi: 10.1007/s13770-019-00198-8

Figure Lengend Snippet: List of TaqMan ® gene expression assay mix (20x)/PCR primers used in RT-qPCR studies

Article Snippet: The conditions for the reactions were set at 95 °C for 20 s and 40 cycles at 95 °C for 1 s and 60 °C for 20 s. The cycle threshold values were determined automatically by the Applied Biosystems StepOnePlus Real-Time PCR System, and the fold-change in the gene expression was calculated by the 2 −ΔΔCT method. table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Gene symbol Gene name Assay ID Species Amplicon length TPO Thyroid peroxidase Hs00892519_m1 Human 53 TG Thyroglobulin Hs00174974_m1 Human 69 TGFB Transforming growth factor beta 1 Hs00998133_m1 Human 57 IL6 Interleukin 6 Hs00985639_m1 Human 66 EGF Epidermal growth factor Hs01099999_m1 Human 70 Open in a separate window List of TaqMan ® gene expression assay mix (20x)/PCR primers used in RT-qPCR studies Fabrication of cell sheets Human primary thyroid cell sheets were constructed using TRCDs.

Techniques: Gene Expression, Amplification

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Fabrication of Functional Cell Sheets with Human Thyrocytes from Non-Tumorous Thyroid Tissue

doi: 10.1007/s13770-019-00198-8

Figure Lengend Snippet: Changes in the gene expression of thyroid cell markers and cytokines. A-E RT-qPCR analyses of TPO, TG, IL-6, TGFB and EGF for cells cultured in DMEM and HDM. GAPDH was set as the housekeeper gene. TPO: thyroid peroxidase; TG: thyroglobulin; TGFB: transforming growth factor-β; EGF: epidermal growth factor. *p < 0.05, **p < 0.01, **p < 0.01, ***p < 0.001 (two-way ANOVA), n.s.: non significance, n = 6 in 2 cases

Article Snippet: The conditions for the reactions were set at 95 °C for 20 s and 40 cycles at 95 °C for 1 s and 60 °C for 20 s. The cycle threshold values were determined automatically by the Applied Biosystems StepOnePlus Real-Time PCR System, and the fold-change in the gene expression was calculated by the 2 −ΔΔCT method. table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Gene symbol Gene name Assay ID Species Amplicon length TPO Thyroid peroxidase Hs00892519_m1 Human 53 TG Thyroglobulin Hs00174974_m1 Human 69 TGFB Transforming growth factor beta 1 Hs00998133_m1 Human 57 IL6 Interleukin 6 Hs00985639_m1 Human 66 EGF Epidermal growth factor Hs01099999_m1 Human 70 Open in a separate window List of TaqMan ® gene expression assay mix (20x)/PCR primers used in RT-qPCR studies Fabrication of cell sheets Human primary thyroid cell sheets were constructed using TRCDs.

Techniques: Gene Expression, Quantitative RT-PCR, Cell Culture